ha cd44 interaction inhibitor 2  (MedChemExpress)

Journal: Bioactive Materials

Article Title: Hyaluronan regulates the assembly structure and biofunction of polycationic silk fibroin to boost microfracture

doi: 10.1016/j.bioactmat.2025.11.037

Figure Lengend Snippet: (A) Macrophages polarization and representative CD206/CD86 IF images. (B) The Elisa test results of IL-1β, TNF-α, TGF-β1, and IL-10. (C) Macrophage polarization under different treatment conditions by flow cytometric. (D) Results of M2 macrophage polarization following treatment with materials. (E) Results of M2 macrophage polarization treated with materials and/or Infliximab/HA-CD44 inhibitor 2. (F) Schematic of collecting joint synovial fluid for Elisa test. (G) The Elisa test results of IL-1β and TNF-α in the synovial fluid. (H) Representative CD86 IF images in cartilage defect. (I) Fluorescence intensity statistics of CD86. n = 3. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: HA-CD44 inhibitor 2 and Infliximab were purchased from MedChemExpress LLC.

Techniques: Enzyme-linked Immunosorbent Assay, Fluorescence

Journal: Bioactive Materials

Article Title: Microfluidic chip-integrated vascularized endometrial complexes: Mitochondrial function and paracrine crosstalk enhance regenerative potential

doi: 10.1016/j.bioactmat.2025.08.035

Figure Lengend Snippet: Endothelial cells promote mitochondrial function and extracellular matrix gene expression of endometrial cells within the dynamically cultured HEO complex.a. Comparison of expression levels of MT-RNR1, MT-RNR2, and MT-ND4L genes between the dynamically cultured EO and HEO complexes in different clusters.b. EEOs were isolated from the dynamically cultured EO and HEO complexes using a mouth pipette. Comparison of MTRNR1, MTRNR2, and MT-ND4L genes in EO and HEO complexes. GAPDH was used as the internal control. ∗∗P < 0.01, ∗∗∗P < 0.001, Student's t-test.c. Flow cytometry analysis showing JC-1 aggregates of mitochondria in the dynamically cultured EO and HEO complexes.d. Ultrastructure of mitochondrion in the dynamically cultured EO and HEO complexes by transmission electron microscopy. Triangle, nucleus; arrow, mitochondrion. Scale bar: 5 μm.e. Flow cytometry analysis of mitochondrial JC-1 aggregates in the dynamically cultured EO and HEO complexes. In the HEO complex, inhibitors of BMP6 (dorsomorphin, 10 μM) and CD44 (HA-CD44, 10 μM) were used to inhibit the respective receptors of EEOs. In the EO complex, recombinant proteins BMP6 (50 ng/mL) or Galectin-9 (10 ng/mL) were introduced as ligands.f. Quantitative results of flow cytometry assays in panel e. ∗P < 0.05, ∗∗∗P < 0.001, one-way ANOVA.g. The electrical resistance, measured using the EVOM2 voltohmmeter in Transwell inserts, reflects the growth of cells in HEO and EO complexes treated with inhibitors and recombinant proteins, respectively. ∗∗∗P < 0.001, two-way ANOVA.h. Comparison of the gene expression level of MMPs (MMP1 and MMP3) and collagens (COL1A1, COL4A1 and COL5A3) between the dynamically cultured EO and HEO complexes in different clusters.i. Gene expression levels of MMP1 and MMP3 in EEOs isolated from the dynamically cultured HEO complex relative to that from the dynamically cultured EO complex. 18S was used as internal control. ∗∗∗P < 0.001, Student's t-test.j. MMP and collagen expression in EO and HEO complexes under estrogen treatment. Left: Representative western blots of MMP1, MMP3, COL1A1, and COL4A1. β-ACTIN was used as loading control. Right: Quantitative analysis of protein expression levels (normalized to β-ACTIN), measured using ImageJ software.Data presented as mean ± SD; ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 (Student's t -test or one-way ANOVA).

Article Snippet: Briefly, the dynamically cultured HEO and EO complexes were cultured in eCHIP for 3 days with treatments including dorsomorphin (10 μM, HY-13418, MCE), HA-CD44 (10 μM, HY-149897, MCE), BMP-6 (50 ng/mL, HY-P700029AF, MCE), Galectin-9 (10 ng/mL, C808, Novoprotein, Jiangsu, China), or DMSO (PWL064, Meilunbio) as a negative control.

Techniques: Gene Expression, Cell Culture, Comparison, Expressing, Isolation, Transferring, Control, Flow Cytometry, Transmission Assay, Electron Microscopy, Recombinant, Western Blot, Software